In Vitro Fertilization (IVF) is the most widely known method in the area of Medically Assisted Reproduction and involves the fertilization of the ovum (egg) and the incubation of the embryo that are produced in laboratory conditions. The process is separated into various stages that start by investigating the existing fertility through a complete testing of both the female and the male factors as well as blood and hormonal check-up. Next comes the ovarian stimulation through medication that leads to embryo transfer, which is in fact, the completion of the procedure.
The fertilization of the eggs is either done with the method of the classic In Vitro Fertilization (IVF) or with the method of micro-insemination Intracytoplasmic Sperm Injection (ICSI) depending on the parameters of the semen and the couple’s medical record.
Fertilization Method IMSI
It is an improvement of the technique of Micro-insemination (ICSI) where morphologically selected spermatozoa are injected during the fertilization (Intracytoplasmic Morphologically-selected Sperm Injection – IMSI).
It is used in cases of severe astheno-terato-spermia where in the man’s sperm sample there are many spermatozoa with deviant morphology. With the help of special lenses high definition microscopy is achieved as well as enlargement of the image of the spermatozoa at about 6500 times. Therefore, we have detailed illustration of their head and morphology and only the best sperms in terms of morphology are used for the egg fertilization.
It is a way of fertilization based on the sperm’s and oocyte’s physiology, hence the name Physiological ICSI (PICSI).
Its difference with ICSI is in the use of special plates and culture mediums where small quantities of processed sperm sample of the husband are placed.
In this material the binding of immature sperm forms is impossible because they do not have the suitable receptors. On the contrary, spermatozoa with distinctly limited chromosome aberrations and significant improvement of their nucleus’ maturation are bound and selected for fertilization.
In each technique (IMSI or PICSI), used either separately or in combination, we select mature and morphologically normal spermatozoa.
Especially for the PICSI method, which is used extensively in the IVF Athens Center, there is distinct improvement of the embryo quality, increased chance of implantation, increase on the rate of clinical pregnancies and decrease of the clinical miscarriages.